The potentially insecticidal Narcissus pseudonarcissus lectin demonstrates age-related mitogenicity
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چکیده
منابع مشابه
Comparison of the Polypeptide Complement of Different Plastid Types and Mitochondria of Narcissus pseudonarcissus
The protein complement of chloroplasts, prochromoplasts, chromoplasts, and mitochondria of the daffodil (Narcissus pseudonarcissus) has been investigated comparatively by gel electro phoresis. Mitochondria do not share common proteins with plastids. On the other hand, there is a broad overlap of the protein complement of the three plastid types investigated, in spite of their different fine st...
متن کاملApparent Identity of Chromoplast and Chloroplast DNA in the Daffodil, Narcissus pseudonarcissus
Although chromoplasts and chloroplasts are mor phologically and functionally very different, it has been shown in certain plants and under certain con ditions, that they are interconvertible [see 1 5]. One could then expect such plastids to contain, at least qualitatively, the same plastome. It appears questionable, however, whether this applies to all plastid types. For example, FreyWyssling...
متن کاملCarbohydrate-binding specificity of the daffodil (Narcissus pseudonarcissus) and amaryllis (Hippeastrum hybr.) bulb lectins.
The carbohydrate binding specificity of the daffodil (Narcissus pseudonarcissus; NPA) and amaryllis (Hippeastrum hybr.; HHA) lectins, isolated from extracts of their bulbs by affinity chromatography on immobilized mannose, was studied by quantitative precipitation, sugar hapten inhibition, and affinity chromatography on the immobilized lectins. These lectins gave strong precipitation reactions ...
متن کاملIdentification of genes associated with perianth senescence in Daffodil (Narcissus pseudonarcissus
We used a PCR-based subtractive procedure to isolate genes that increased in abundance in the perianth of incipiently senescent daffodil (Narcissus pseudonarcissus L. ‘Dutch Master’) flowers. Using RNA gel blot analysis we confirmed that 18 of the transcripts were senescence-associated. A number of these have homologs in previously-studied senescing floral and leaf tissue systems (cysteine prot...
متن کاملEngineering a Therapeutic Lectin by Uncoupling Mitogenicity from Antiviral Activity
A key effector route of the Sugar Code involves lectins that exert crucial regulatory controls by targeting distinct cellular glycans. We demonstrate that a single amino-acid substitution in a banana lectin, replacing histidine 84 with a threonine, significantly reduces its mitogenicity, while preserving its broad-spectrum antiviral potency. X-ray crystallography, NMR spectroscopy, and glycoclu...
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ژورنال
عنوان ژورنال: FEMS Immunology and Medical Microbiology
سال: 2002
ISSN: 0928-8244
DOI: 10.1016/s0928-8244(02)00271-7